Agricultural tourism (agritourism) has become an emerging development pathway in the Mekong Delta, where agriculture, culture, and water-based ecosystems intersect to form distinctive rural landscapes. Within Vietnam’s broader agenda of rural restructuring and sustainable development, agritourism in the region has gradually evolved from small-scale household initiatives into a more organized sector shaped by the interaction of state policies, enterprise strategies, and community participation. This study examines how these three actors collectively influence agritourism development in the Mekong Delta. Using a qualitative research design and an embedded case study approach, the analysis draws on national and provincial policy documents, industry reports, and representative agritourism models such as My Khanh Tourist Village, Con Son Community Cooperative, Con Chim Ecotourism Site, Con Ong experiential farm, and the Dinh Yen Mat weaving craft village. The findings indicate that since 2010, the Vietnamese government has established a policy framework promoting experiential agriculture-based tourism, regional connectivity, climate-resilient development, and cultural preservation, thereby enabling enterprises to upgrade facilities, diversify tourism services, and expand regional tour circuits. At the same time, local communities have reorganized tourism activities through cooperative and community-based models that integrate agricultural practices, culinary heritage, and craft traditions into visitor experiences. Successful initiatives demonstrate improvements in income generation, employment opportunities, cultural continuity, and environmental awareness. However, agritourism in the Mekong Delta still faces challenges, including fragmented policy implementation, limited destination management capacity, weak interprovincial coordination, repetitive tourism products, and environmental pressures associated with climate change. The study argues that the sustainability of agritourism in the region depends on strengthening the alignment between policy frameworks, enterprise innovation, and communitybased stewardship.