In recent years, asymmetric gold nanoparticles have attracted a lot of attention from researchers owing to their unique properties and varied applications in many fields. In this study, gold nanobranches were prepared using a one-step, green reducing method, with the HEPES buffer acting as both a reducing agent and surfactant. The formation of gold nanoparticles was evaluated using UV-Vis spectroscopy by controlling several practical factors, including the volume of gold salt precursor, the concentration of HEPES buffer, and the solution pH. The morphologies and crystallization of the gold nanobranches were characterized by Scanning electron microscopy (SEM) and X-ray diffraction (XRD). The results indicated that under the optimal synthesis conditions, namely 250 µL of 5 mM HAuCl₄, 0.10 M HEPES, and a pH of 7.5, most of the gold particles in the colloidal solution exhibited multiple branches, with an average size ranging from 20 to 35 nm and high crystal density. This study presented a simple synthesis method utilizing eco-friendly substances to replace conventional reducing agents, contributing to the sustainable development of nanotechnology.