The callus is a disorganized mass of cells that grow in plants in response to various biotic and abiotic stimuli. Callus plays an important role in cell cultures in vitro, as a starting material for many subsequent studies such as single-cell culture, protoplast culture, micropropagation, etc. In this review, callus as well as the factors affecting callus formation and proliferation (plant growth regulator, mineral medium, carbon source, type of explant, light condition, callus line) are explored to provide a brief overview of callus culture

The papain-like protease (PLPro) is a highly conserved, non-structural protein that plays a crucial role in the formation of the replication-transcription complex and the processing of polyproteins in SARS-CoV-2, as well as improving the host’s antiviral immune responses against said virus. Chalcone is a common ingredient, which can be found in a multitude of natural substances, such as food and herbs. It has been proven to have various biological activities, including antiviral effects. Previous studies have identified several natural chalcone-based compounds with the ability to inhibit SARS-CoV-2 by targeting the PLPro enzyme. Based on these findings, this study investigated potential chalcone-derived PLPro inhibitors, as retrieved from Pubchem and in-house libraries. Virtual screening protocols, specifically molecular docking and molecular dynamics simulating filter, were applied to reach the desired goal. As a result, 1448 out of 1454 chalcone derivatives can effectively bind to SARS-CoV-2 via PLPro. The 5 substances with the most suitable docking score and binding mode were selected for the next step. Through MD, CID1021201513 and CID101585417 showed the greatest potential in targeting PLPro. However, further in vitro and in vivo studies must be conducted before the bio-activities of these chalcones against SARS-CoV-2 can be confirmed. Furthermore, the ligand-protein interaction mode analysed in this research can help design effective chalcone derivatives.

The Stripe ebony tree (Diospyros frutescens Blume) is considered a species in danger of extinction due to overexploitation. Therefore, there is an urgent need to regenerate and conserve the species for future generation. In this study micropropagation protocol of Diospyros frutescens Blume was developed using nature zygotic embryos. Zygotic embryos isolated from germinated on MS medium containing 0.4mg L-1 Kinetin + 1.0mg L-1 BA. There were (83.11±0.79%) of explants induced shoots after 30 days of culture. The highest number of shoot on 1/2 MS medium supplemented with 2.5mg L-1 BA + 0.6mg L-1 IBA was 5.84±0.035 shoots per explant after 45 days of culture. The percentage of shoot produced roots the highest 85.52±0.48% after 45 days culture on 1/2 MS medium contain 0.8mg L-1 IBA

Ficus hispida L.f. (FH) is among the widely cultivated tree species in Asian regions. The aim of this work was to determine phenolic and flavonoid contents, antioxidant, in vitro anti-inflammatory activities of FH extracts prepared with methanol (ME), ethanol (ET) and ethyl acetate (EA). The extract obtained from ethyl acetate contained the highest total phenolic and flavonoid contents (92.75 +/- 5.14 mg GAE/g and 5.67 +/- 0.65 mg QE/g), followed by ME and ET. The capacity to scavenge ABTS free radical of the extracts followed the order: EA > ME > ET while their DPPH activity differed insignificantly. The inhibitory effect of EA and ME (IC¬50 = 362.84 +/- 29.05 and 386.95 +/- 11.10 ug/mL, respectively) were higher than that of ET. Correlation analysis showed a high positive correlation between total phenolic content and ABTS activity. The findings of the study would be useful for development of new nature-derived agents for prevention and treatment of diseases.

Diabetes affected about 537 million adults aged 20 to 79 in 2021. Higher vegetable intake helps to manage type 2 diabetes mellitus. Thus, this research aimed to recognize and record the vegetable species currently sold in Sri Lanka's Vavuniya District. At least three fieldwork visits were made between January 2019 and January 2022 to each of the Vavuniya District's major market sites, as well as the neighboring vegetable shops and street stalls. Until June 2022, published relevant research papers were searched in the Web of Science, PubMed, and ScienceDirect databases for the recorded vegetable species in this study to assess the antidiabetic scientific proof. This study recognized and recorded 72 vegetable species from 25 families. Consequently, a study of the literature on 65 vegetable species found that most of the vegetable species had in vivo (37%), followed by clinical (15%), and in vitro (14%) scientific evidence, and six antidiabetic active substances were isolated from the analyzed vegetable species. This work establishes the foundation for additional studies on the fruit species traded in Vavuniya District.

Indigofera aspalathoides Vahl ex DC. belongs to the Fabaceae family. I. aspalathoides is applied to heal tumors, inflammations, diabetes, leprosy, and kidney illnesses in traditional medicines. Compounds including kaempferol, kaempferol 5-O-β-D-glucopyranoside, 5,4'-dihydroxy 6,8-dimethoxy 7-O-rhamnosyl flavone, indigocarpan, and mucronulatol have isolated from this plant species. Hitherto, there is no comprehensive review available regarding the reported bioactivities of I. aspalathoides. Thus, this article goals to analyze, summarize and document the published bioactivities-related publications. Electronic databases the Web of Science, Scopus, ScienceDirect, and PubMed used to find relevant publications from 1900 to December 2020. Thus far, only in vivo and in vitro scientific evidence levels of bioactivities are available. I. aspalathoides holds such as anti-inflammatory, anticancer, antihepatotoxic, anti-arthritic, immunomodulatory, and antidiabetic properties. Overall, immunomodulatory, anti-inflammatory, and anticancer compounds have been isolated from this plant species Therefore, additional bioactivity and phytochemical-related researches would need to perform to generate more scientific evidence for other applications. This work will be useful for further bioactivity and phytochemical studies using this plant species.