Pain is a global health concern that requires the attention of the healthcare services. This study aimed to evaluate nurses’ knowledge, skills on pain management for caring post – transurethral resection of the prostate (TURP) as well as explore the relationship between knowledge and skill regarding pain management for post – TURP patients. This study was conducted on 230 nurses who have worked at two public hospitals in Ho Chi Minh city from April to June 2024. The result showed that the majority of nurses were female, had a mean age of 37.3 years and an average of 13.7 years of direct clinical experience. Of these, 57.4% of nurses have attended the previous pain training course. The majority of nurses lack knowledge regarding pain management with 86.5%. The study also indicated that nurses’ educational level, knowledge and attitude toward pain management are significant predictive factors of nurses’ skills on pain management when caring post – TURP patients. Knowledge on pain management had a very strong positive correlation with attitude (r = 0.820, p < 0.001) as well as skills (r = 0.91, p < 0.001). Besides, there was a very strong and positive correlation between attitude and skills on pain management (r = 0.91, p < 0.001). There was a mediating relationship between knowledge, attitude and skills on pain management, attitude played a partial mediating role between knowledge and skills. In summary, the study indicates a need for targeted educational interventions to bridge the gap between knowledge and skills in pain management, particularly in non-pharmacological strategies, to improve overall care for post-TURP patients.